**Question:** A 28 years old lady is suspected to have polycystic ovarian disease. Sample for testing LH & FSH are best taken on the following days of menstrual cycle:

A. Day 2-3
B. Day 8-10
C. Day 14-15
D. Day 21

**Core Concept:**

Polycystic Ovarian Syndrome (PCOS) is a hormonal disorder characterized by the presence of multiple small cysts in the ovaries, irregular menstrual cycles, and elevated levels of LH (Luteinizing Hormone) and FSH (Follicle-Stimulating Hormone). Testing these hormones requires the appropriate timing of sample collection to accurately reflect the hormonal milieu.

**Why the Correct Answer is Right:**

The best time to collect samples for LH and FSH testing in a patient suspected of having PCOS is during the follicular phase of the menstrual cycle. In this phase, LH and FSH levels are highest and lowest respectively.

**Why Each Wrong Option is Incorrect:**

A. Day 2-3: This is too early in the menstrual cycle, as LH and FSH levels are still rising during the follicular phase.
B. Day 8-10: Though the cycle is advancing, this option still does not ensure the highest LH and lowest FSH levels during the follicular phase.
C. Day 14-15: This is the mid-cycle phase, which is too late to accurately assess the hormonal milieu during the follicular phase.
D. Day 21: This is the luteal phase, when LH levels are decreasing and FSH levels are increasing, making this option unsuitable for accurate hormonal assessment.

**Clinical Pearl:**

For accurate hormonal assessment in patients suspected of having PCOS, collect blood samples during the follicular phase, typically on days 2-3. This ensures that LH levels are high and FSH levels are low, providing a clearer understanding of hormonal imbalances in the patient.