Isoelectric point of a protein is located by which method?
Correct Answer: Isoelectric cardiac failure
Description: Ans. is 'b' i.e.,Isoelectric cardiac failure Isoelectric focusing is a procedure used to determine the isoelectric point (pI) of a protein.A pH gradient is established by allowing a mixture of low molecular weight organic acids and bases to distribute themselves in an electric field generated across the gel.When a protein mixture is applied, each protein migrates until it reaches the pH that matches its pI.Proteins with different isoelectric points are thus distributed differently throughout the gel.
Category:
Biochemistry
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