Biochemistry Irreversible steps of Glycolysis are catalyzed by: A Hexokinase, Phospho Glycerate Kinase, Pyruvate Kinase B Pyruvate Kinase, Fructose 1,6 Bisphosphatase, Phosphofructokinase C Hexokinase, Phosphofructokinase, Pyruvate Kinase D Glucokinase, Pyruvate Kinase, Glyceraldehyde 3 Phosphate Dehydrogenase 💡 Explanation Irreversible steps of Glycolysis are catalyzed by: ✓ Correct Answer: C. Hexokinase, Phosphofructokinase, Pyruvate Kinase