Biochemistry Irreversible steps of Glycolysis are catalysed by: A Hexokinase, Phosphofructokinase, Pyruvate Kinase B Glucokinase, Pyruvate Kinase, Glyceraldehyde 3 Phosphate Dehydrogenase C Hexokinase, Phospho Glycerate Kinase, Pyruvate Kinase D Pyruvate Kinase, Fructose 1,6 Bisphosphatase, Phospho FructoKinase 💡 Explanation Irreversible steps of Glycolysis are catalysed by: ✓ Correct Answer: A. Hexokinase, Phosphofructokinase, Pyruvate Kinase