Introduction of DNA with the help of electricity is
**Question:** Introduction of DNA with the help of electricity is
A. Electroporation
B. Electrofusion
C. Electromigration
D. Electrofusion
**Correct Answer:** A. Electroporation
**Core Concept:** Electroporation is the process of introducing genetic material into cells using electric pulses. This technique is particularly useful for gene therapy and DNA vaccination.
**Why the Correct Answer is Right:**
Electroporation is the correct answer because it involves applying short, high-intensity electric pulses to the cells, causing pores to form in the cell membrane. These pores allow the genetic material (DNA or RNA) to enter the cell, thereby introducing the genetic material into the cell. This technique is non-invasive, selective, and efficient, making it a popular choice for genetic manipulation.
**Why Each Wrong Option is Incorrect:**
A. Electrofusion (option D) is a related technique but involves the fusion of two cells using electric pulses, whereas electroporation focuses on introducing genetic material into a single cell.
B. Electromigration (option C) is the movement of charged particles due to an electric field, not a method for introducing genetic material into cells.
D. Electrofusion (option D) is similar to electrofusion but specifically refers to the fusion of cells, not the introduction of genetic material into a single cell as in electroporation.
**Clinical Pearl / High-Yield Fact:** Electroporation has significant implications in genetic engineering, gene therapy, and DNA vaccination, making it an essential tool in the field of molecular biology and medicine.