Cell fusion is an innovative technique of preparing specific antibodies. Techniques to induce the cell-fusion include the following except
**Core Concept**
Cell fusion is a process where two cells combine to form a single cell, often used in immunology to produce specific antibodies. This technique involves the fusion of antibody-secreting B cells with myeloma cells to create hybridoma cells, which can produce large quantities of monoclonal antibodies.
**Why the Correct Answer is Right**
The correct answer is the technique that does not induce cell fusion. Cell fusion can be induced by various methods, including the use of Sendai virus, polyethylene glycol (PEG), and electrofusion. These methods create pores in the cell membrane, allowing the cells to fuse together.
**Why Each Wrong Option is Incorrect**
**Option A:** Sendai virus is a correct method of inducing cell fusion, as it causes the cell membrane to merge, allowing the cells to fuse.
**Option B:** Polyethylene glycol (PEG) is also a correct method of inducing cell fusion, as it creates pores in the cell membrane, allowing the cells to fuse.
**Option C:** Electrofusion is another correct method of inducing cell fusion, as it uses electrical pulses to create pores in the cell membrane, allowing the cells to fuse.
**Clinical Pearl / High-Yield Fact**
It's essential to note that cell fusion is a critical technique in biotechnology, enabling the production of monoclonal antibodies with specific antigen-binding properties.
**Correct Answer:** D.