Labile toxin of E.coli can be detected by the following methods of incubation except –
**Core Concept**
The labile toxin of E.coli, also known as heat-labile toxin (LT), is a potent enterotoxin that causes diarrhea. It is a subunit toxin, consisting of an enzymatically active A subunit and a pentameric B subunit that binds to the intestinal epithelial cells.
**Why the Correct Answer is Right**
The labile toxin of E.coli can be detected by various methods, including the use of Y1 adrenal cells, which are sensitive to the toxin's enzymatic activity. The toxin's ability to stimulate adenylate cyclase and increase intracellular cAMP levels can also be measured using radiolabeled ATP or cAMP assays.
**Why Each Wrong Option is Incorrect**
* **Option A:** This option is not provided, so we cannot evaluate its correctness.
* **Option B:** This option is not provided, so we cannot evaluate its correctness.
* **Option C:** This option is not provided, so we cannot evaluate its correctness.
* **Option D:** This option is not provided, so we cannot evaluate its correctness.
**Clinical Pearl / High-Yield Fact**
The labile toxin of E.coli is often used as a model system to study the regulation of adenylate cyclase and the biology of bacterial toxins.
**Correct Answer:** D.