Biochemistry An enzyme increases the rate of a chemical reaction through which one of the following effects? A Decreasing the free energy of the substrate B Increasing the free energy of the product C Decreasing the free energy of the transition state D Shifting the equilibrium of the reaction toward the product 💡 Explanation An enzyme increases the rate of a chemical reaction through which one of the following effects? ✓ Correct Answer: C. Decreasing the free energy of the transition state