Inclusion bodies of trachoma are stained with?
**Core Concept**
Trachoma is a bacterial infection caused by Chlamydia trachomatis, leading to the formation of characteristic inclusion bodies within epithelial cells. The identification of these inclusion bodies is crucial for the diagnosis of trachoma.
**Why the Correct Answer is Right**
The inclusion bodies of trachoma are stained with Giemsa stain, which is a type of Romanowsky stain. This staining technique allows for the visualization of the characteristic "owl's eye" appearance of the inclusion bodies, which are formed by the accumulation of chlamydial elementary bodies within the host cell. The Giemsa stain is particularly effective in highlighting the inclusion bodies due to its ability to differentiate between the chromatin and the cytoplasm of the host cell.
**Why Each Wrong Option is Incorrect**
* **Option A:** Wright's stain is typically used for staining blood films and is not suitable for staining inclusion bodies of trachoma.
* **Option B:** Gram stain is used for identifying bacteria based on their cell wall composition, but it is not effective for staining inclusion bodies.
* **Option C:** Hematoxylin and eosin (H&E) stain is a general-purpose stain used for histopathological examination, but it is not specific for staining inclusion bodies of trachoma.
**Clinical Pearl / High-Yield Fact**
It's essential to remember that the diagnosis of trachoma requires a combination of clinical evaluation, laboratory testing, and histopathological examination. The presence of inclusion bodies in conjunctival scrapings or biopsy specimens is a key diagnostic feature of trachoma.
**Correct Answer:** C.