In which type of chromatography, the proteins are bound to another substance?
Correct Answer: Affinity chromatography
Description: Affinity chromatography is based on the exceptional ability of biologically active substances to bind specifically and reversibly other substances generally called ligands or affinity ligands or simply affinants. Affinity chromatography provide a high degree of protein purification due to the specificity of the binding step. Three most commonly used column chromatography methods are ion exchange chromatography, gel filtration and affinity chromatography. Ion exchange chromatography uses electrostatic interactions between the protein and the solid matrix to fractionate proteins.Gel filtration: It separates protein molecules by size. This method depends on special beads that permit small proteins to penetrate into their interior while excluding large proteins from this region.Ref: Affinity Chromatography By J. Turkova page 7, Principles of Molecular Biology: Genes to Proteins By Buon E. Trop.
Category:
Biochemistry
Get More
Subject Mock Tests
Practice with over 200,000 questions from various medical subjects and improve your knowledge.
Attempt a mock test nowMock Exam
Take an exam with 100 random questions selected from all subjects to test your knowledge.
Coming SoonGet More
Subject Mock Tests
Try practicing mock tests with over 200,000 questions from various medical subjects.
Attempt a mock test now