Gynaecology & Obstetrics In triple screening test for Down’s syndrome during pregnancy all of the following are included except. A Serum beta hCG B Serum oestriol C Maternal serum Alfa fetoprotein D Acetyl cholinesterase 💡 Explanation In triple screening test for Down's syndrome during pregnancy all of the following are included except. ✓ Correct Answer: D. Acetyl cholinesterase