Pathology In the coagulation cascade the clot is initiated by negatively charged paicles on the glass bead. It is in-vivo initiated by: A Tissue factor B Thrombin C Endothelin D vWF 💡 Explanation In the coagulation cascade the clot is initiated by negatively charged paicles on the glass bead. It is in-vivo initiated by: ✓ Correct Answer: A. Tissue factor