In PCR Acquaticus thermophilus is preferred over E.coli. because:
## Core Concept
The question pertains to the preference for *Thermus aquaticus* over *E. coli* in the Polymerase Chain Reaction (PCR) technique. This involves understanding the role of thermostable DNA polymerases in PCR.
## Why the Correct Answer is Right
*Thermus aquaticus*, a thermophilic bacterium, produces a DNA polymerase known as Taq polymerase. This enzyme is **thermostable**, meaning it can withstand the high temperatures required for the denaturation step of PCR without losing its activity. In contrast, *E. coli* DNA polymerase, derived from *E. coli*, is not thermostable and would denature and become inactive at the high temperatures used in PCR. The thermostability of Taq polymerase allows for the automation of PCR, making it a crucial component for this technique.
## Why Each Wrong Option is Incorrect
- **Option A:** This option is blank and does not provide a reason.
- **Option B:** This option is also blank and does not offer a valid reason.
- **Option C:** Similarly, this option is blank.
- **Option D:** This option is blank as well.
## Clinical Pearl / High-Yield Fact
A key point to remember is that the use of *Thermus aquaticus*-derived Taq polymerase is what enables the PCR process to be cycled through high temperatures repeatedly without the need for replenishing the enzyme. This has revolutionized genetic engineering and diagnostics.
## Correct Answer: D. Taq polymerase is thermostable.