In molecular cloning, Blue-white screening is used for –
Correct Answer: To identify desired chromosomal DNA insert in plasmid vectors
Description: Screening for selecting the cells with vector DNA containing the desired insertional DNA sequence can be done by :
Antibiotic resistance markers:- Allow only cells in which the vector has been transfected to grow.
Blue-white screening:- It is based on lac operon. The hydrolysis of X-gal by β-galactosidase cause blue colour in colonies & indicates that colonies contain the unligated vector. Whereas, white colonies indicate insertion of foreign DNA and loss of cells ability to hydrolyse marker.
PCR, restriction fragment analysis and DNA sequencing:- Confirmatory methods.
Category:
Biochemistry
Get More
Subject Mock Tests
Practice with over 200,000 questions from various medical subjects and improve your knowledge.
Attempt a mock test nowMock Exam
Take an exam with 100 random questions selected from all subjects to test your knowledge.
Coming SoonGet More
Subject Mock Tests
Try practicing mock tests with over 200,000 questions from various medical subjects.
Attempt a mock test now