In CRISPR-Cas 9 system, which repair mechanism is used for genome editing?
Correct Answer: Non homologous end repair
Description: * CRISPR-Cas-9 endonuclease system introduces double stranded breaks in DNA at the specific target site. Now cell tries to repair this double stranded breaks by two mechanism: 1. Non-homologous end repair 2. Homologous repair * Non-homologous repair mechanism is always a possibility as it does not require homologous DNA. * The homologous repair will only work if the DNA homologous to the cut DNA is present but homologous DNA is not always present or available. So, if both options are given, best option is Non-homologous repair.
Category:
Biochemistry
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