Microbiology In autoclave, the temperature is____ for a hold time of_____ at 15psi atmospheric pressure. A 121degC for a hold time of 10 minutes B 121degC for a hold time of 15 minutes C 100degC for a hold time of 30 minutes D 100degC for a hold time of 60 minutes 💡 Explanation In autoclave, the temperature is____ for a hold time of_____ at 15psi atmospheric pressure. ✓ Correct Answer: B. 121degC for a hold time of 15 minutes