In a sample of dsDNA, the molar ratio of Adenosine is 20%. From this information, tell the content of Cytosine?
Correct Answer: 30
Description: According to chargaff's rule in a ds DNA, the total purine content is equal to the pyrimidine content. Since =, so = 20% + = 20+20 = 40% + = 100-(+)= 100-40% =60% As = , so = 60/2 = 30% Chargaff Rule 1. Total number of purine (A+G) is equal to total number of pyrimidine (C+T) I.e. A + G / C + T = I 2. A = T or A/T = I 3. G= C or G/C = I 4. The numbers of (A+T) and (G+C) are only varibales, i.e., A + T [?] G + C If (A+T) > (G+C) then DNA is referre as (AT-type). If (G+C) > (A+T) then DNA is referred as (GC-type). Chargaff's Rule only applies to double-stranded DNA, due to the complementary base pairing that occurs between A-T and C-G.
Category:
Biochemistry
Get More
Subject Mock Tests
Practice with over 200,000 questions from various medical subjects and improve your knowledge.
Attempt a mock test nowMock Exam
Take an exam with 100 random questions selected from all subjects to test your knowledge.
Coming SoonGet More
Subject Mock Tests
Try practicing mock tests with over 200,000 questions from various medical subjects.
Attempt a mock test now