The clinical picture is suggestive of Chlamydial infection hence the most appropriate culture medium is McCoy cells(human synol carcinoma cell line). Abnormal urethral Gram's stains showing >10 PMNs/1000A~ field in women with dysuria but without coliform bacteriuria suppo the diagnosis of chlamydial urethritis. Thayer-Main agar: Neisseria Robeson's cooked meat: Anaerobes, especially Clostridia Pleuropneumonia-like organisms (PPLO) broth: Mycoplasma spp. and Ureaplasma spp. Reference: Ananthanarayan and Paniker&;s Textbook of Microbiology Tenth edition