A rapid method of chromosome identification in interphase is –
## Core Concept
The question tests knowledge of cytogenetic techniques used for chromosome identification, particularly in the interphase stage of the cell cycle. Interphase is the stage where the cell grows, replicates its DNA, and prepares for cell division, and chromosomes are not condensed and thus not easily identifiable under a microscope. Techniques that can identify chromosomes in this stage are crucial for genetic diagnostics.
## Why the Correct Answer is Right
The correct answer, , involves the use of **Fluorescence In Situ Hybridization (FISH)**. FISH is a laboratory technique used to detect and locate specific DNA sequences on chromosomes. It involves the use of fluorescent probes that bind to specific parts of the chromosome. This technique can be used on interphase cells because it can detect specific DNA sequences regardless of the cell cycle phase. FISH is widely used for genetic testing, including the identification of chromosomal abnormalities such as deletions, duplications, and translocations.
## Why Each Wrong Option is Incorrect
- **Option A:** This option is incorrect because conventional karyotyping requires cells to be in metaphase, where chromosomes are condensed and can be easily visualized and analyzed. This does not allow for rapid identification in interphase.
- **Option B:** This option is incorrect as it does not specify a known technique related to chromosome identification in interphase.
- **Option D:** This option is incorrect because, similar to conventional karyotyping, this would not be applicable for rapid identification in interphase.
## Clinical Pearl / High-Yield Fact
A key point to remember is that **FISH** is particularly useful for its ability to analyze chromosomes in **interphase cells**, making it a powerful tool for prenatal diagnosis, cancer genetics, and research. This technique's application in clinical settings has significantly improved the diagnosis and understanding of genetic disorders.
## Correct Answer Line
**Correct Answer: C. FISH**.