Gram staining sequence is

A. Carbol fuchsin – Iodine – Acetone – Methyl violet

B. Methyl violet – Iodine – Acetone – Carbol fuchsin

C. Methyl violet – Acetone – Iodine – Carbol fuchsin

D. Carbol fuchsin – Acetone – Iodine – Methyl violet

Correct Answer: Methyl violet – Iodine – Acetone – Carbol fuchsin

Description: Primary stain – Crystal violet or Methyl violet Mordant – Gram's iodine Decolorizer – Acetone or Ethanol Secondary stain – Safranin or Carbol fuchsin

Category: Microbiology

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