Glassware sterilization is done by?
**Core Concept**
Autoclaving is a widely used method for sterilizing equipment and glassware in medical settings. This process involves subjecting the items to high-pressure steam, which is lethal to microorganisms.
**Why the Correct Answer is Right**
Autoclaving uses steam at 121°C (250°F) and 15 psi to denature proteins, disrupt cell membranes, and ultimately kill bacteria, viruses, and spores. The steam penetrates the equipment and glassware, ensuring thorough sterilization. Autoclaving is a reliable and efficient method, making it the preferred choice for laboratory and hospital settings.
**Why Each Wrong Option is Incorrect**
**Option A:** Dry heat sterilization, while effective, is slower and less commonly used for glassware sterilization. It involves heating items to 160-170°C (320-338°F) for several hours.
**Option B:** Ethylene oxide sterilization is typically used for heat-sensitive equipment and is not the primary method for glassware sterilization. It involves exposing items to ethylene oxide gas.
**Option C:** Chemical sterilization, such as using glutaraldehyde or formaldehyde, is not a standard method for glassware sterilization. These chemicals are often used for surface disinfection.
**Clinical Pearl / High-Yield Fact**
Remember that autoclaving is a reliable method for sterilizing glassware, but it's essential to follow proper protocols to ensure effective sterilization, including using a validated autoclave, monitoring temperature and pressure, and verifying sterility through biological indicators or other methods.
**Correct Answer:** A. Autoclaving.