Gynaecology & Obstetrics First histological sign of ovulation is A Vacuoles at apical portion of secretory nonciliated cells. B Cessation of glandular cell mitosis. C Absent glycoprotein in secretory non ciliated cells. D Subnuclear vacuoles and pseudostratification in basal portion of glandular epithelium. 💡 Explanation First histological sign of ovulation is ✓ Correct Answer: D. Subnuclear vacuoles and pseudostratification in basal portion of glandular epithelium.