Enzyme(s) used in polymerase chain reaction is/are –
## Core Concept
The polymerase chain reaction (PCR) is a laboratory technique used to amplify specific segments of DNA. This process involves the use of enzymes that can withstand high temperatures, which are necessary for the denaturation of DNA. The key enzyme used in PCR is a type of DNA polymerase.
## Why the Correct Answer is Right
The correct answer, **Taq polymerase**, is right because Taq polymerase, or Thermus aquaticus DNA polymerase, is a thermostable enzyme that can survive the high temperatures required for PCR. It synthesizes the new DNA strands by adding nucleotides to the template. This enzyme is crucial for the amplification process as it can withstand the repeated heating and cooling cycles of PCR.
## Why Each Wrong Option is Incorrect
- **Option A:** This option is blank and does not provide an enzyme, making it incorrect by default.
- **Option B:** This option is also blank and does not specify an enzyme, which makes it incorrect.
- **Option C:** Similarly, this option does not provide any information about an enzyme, making it incorrect.
- **Option D:** This option is blank as well, which means it does not accurately represent an enzyme used in PCR.
## Clinical Pearl / High-Yield Fact
A key point to remember is that **Taq polymerase** lacks proofreading activity, which means it does not correct mistakes as it synthesizes DNA. This is why PCR products may have a higher error rate compared to DNA synthesis in vivo. However, for many applications, especially when the goal is to amplify a specific sequence rather than obtain a perfectly accurate copy, Taq polymerase is sufficient and widely used.
## Correct Answer: C. Taq polymerase.