Surgery DNA sequencing by the Sanger method takes advantage of which propey of DNA synthesis to generate a sequencing ladder? A Addition of nucleotides requires a free OH group on the 3' end of the DNA strand B DNA has a free 5' phosphoryl group C DNA polymerase has proofreading capabilities D Nucleotides are linked by phosphodiester bonds 💡 Explanation DNA sequencing by the Sanger method takes advantage of which propey of DNA synthesis to generate a sequencing ladder? ✓ Correct Answer: A. Addition of nucleotides requires a free OH group on the 3' end of the DNA strand