**Core Concept**
The differentiation between Neisseria gonorrhoeae and Neisseria meningitidis relies on several key biochemical and serological characteristics. These two species are closely related but exhibit distinct differences in their growth requirements, metabolic profiles, and antigenic properties.

**Why the Correct Answer is Right**
The correct answer is based on the fact that Neisseria gonorrhoeae can be differentiated from Neisseria meningitidis by its inability to grow on selective media containing vancomycin and colistin. This is due to the presence of a unique gene in Neisseria meningitidis that encodes for a vancomycin-resistant enzyme. Additionally, Neisseria gonorrhoeae can be identified by its susceptibility to the antibiotic penicillin, whereas Neisseria meningitidis is resistant to penicillin.

**Why Each Wrong Option is Incorrect**
* **Option A:** This option is incorrect because it does not specify a valid method for differentiating between Neisseria gonorrhoeae and Neisseria meningitidis.
* **Option B:** This option is incorrect because it refers to a characteristic that is not unique to either species.
* **Option C:** This option is incorrect because it is based on a misinterpretation of the biochemical properties of the two species.

**Clinical Pearl / High-Yield Fact**
It is essential to note that both Neisseria gonorrhoeae and Neisseria meningitidis can cause severe infections, and accurate identification is crucial for guiding treatment and preventing transmission.

**Correct Answer:** D.