Cultivation of virus in all of the following media are used for isolation of viruses from clinical samples, EXCEPT:
**Core Concept**
The question is testing knowledge on the various types of cell culture media used for virus isolation from clinical samples. Cell culture is a laboratory technique used to grow cells from various sources, including microorganisms, in a controlled environment. Virus isolation in cell culture is a crucial step in diagnosing viral infections.
**Why the Correct Answer is Right**
The correct answer is **A.** (or the other options if A is incorrect). Virus isolation in cell culture typically involves the use of a suitable cell line that can support the growth of the virus. The most commonly used cell lines for virus isolation are:
* **Option A:** Human epithelial cells (e.g., HEp-2) for respiratory viruses (e.g., influenza, respiratory syncytial virus)
* **Option B:** Vero cells for enteroviruses (e.g., poliovirus, coxsackievirus)
* **Option C:** MRC-5 cells for cytomegalovirus (CMV)
Each of these cell lines is specifically chosen for its ability to support the growth of the virus in question.
**Why Each Wrong Option is Incorrect**
* **Option D:** Lymphocytes are not typically used for virus isolation in cell culture. While lymphocytes can be infected with certain viruses, they are not a suitable cell line for routine virus isolation.
**Clinical Pearl / High-Yield Fact**
When culturing viruses in cell culture, it's essential to choose the correct cell line to ensure successful isolation. The choice of cell line depends on the type of virus being isolated and the specific characteristics of the virus.
**Correct Answer:** D. Lymphocytes.