Conventional cytogenetics are difficult in solid tumors especially in case of Ca Cx due to aEUR’

Correct Answer: Bacterial contamination of the specimen
Description: Bacterial contamination of the specimen Ideally, the answer should be "lack of good metaphase activity". However, if that option is not mentioned, bacterial contamination is the closest. Although we are not sure how bacterial contamination would hamper cytogenetics. But the other options are not correct. Cytogenetics is the study of chromosomal structure. - It can be done by conventional technique like karyotyping or molecular techniques like FISH, sky, multiplex fish, comparative genomic hybridization. "Conventional cytogenetics needs good metaphase activity" -If metaphase is distinct cytogenetics is easy About the option, High mitotic rate If mitotic rate is high, cytogenetics would definitely be easy. Technical difficulties that can affect cytogenetics - Overgrowth of neoplastic cells by reactive nonneoplastic cells - Unpredictable growth of neoplastic cells in tissue culture - Predominance of nonble tumour (necrotic sample) - Contamination of tumour by bacteria or fungi
Category: Pathology
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