In a reaction substrate is available in a concentration that is l000times the Km value of the enzyme. After 9 minutes of reaction, l7o substrate is conveed to product (12 microgram/ml). If the concentration of the enzyme is changed to 1/3 and concentration of substrate is doubled. What is the time taken to conve the substrate into the same amount of product, i.e. I 2microgram/ml?

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Vikas Bhardwaj
Medical Technologist, AIIMS New Delhi
Updated: Apr 17, 2026