Biochemistry During DNA replication, which of the following enzymes produces a sho strand of RNA complementary to the template DNA with a free 3′-OH end? A DNA ligase B Polymerase I C Polymerase III D Primase 💡 Explanation During DNA replication, which of the following enzymes produces a sho strand of RNA complementary to the template DNA with a free 3'-OH end? ✓ Correct Answer: D. Primase