Complementary RNA sequence of 5&; AGTCTGACT3&;
**Core Concept**
The process of determining the complementary RNA sequence involves matching each nucleotide in the original sequence with its corresponding base pair in the complementary sequence, according to the rules of base pairing: adenine (A) pairs with uracil (U), guanine (G) pairs with cytosine (C), and vice versa.
**Why the Correct Answer is Right**
To find the complementary RNA sequence, we will match each nucleotide in the given sequence with its corresponding base pair. The given sequence is 5'-AGTCTGACT-3'. Using the base pairing rules, we get:
- A pairs with U
- G pairs with C
- T pairs with A
- C pairs with G
- T pairs with A
- C pairs with G
- A pairs with U
- C pairs with G
- T pairs with A
So, the complementary RNA sequence is 5'-UACGACTGU-3'.
**Why Each Wrong Option is Incorrect**
**Option A:** This option is incorrect because it does not follow the base pairing rules. For example, the first nucleotide 'A' in the original sequence should pair with 'U' in the complementary sequence, not with any other nucleotide.
**Option B:** This option is incorrect because it does not correctly apply the base pairing rules. For instance, the second nucleotide 'G' in the original sequence should pair with 'C' in the complementary sequence, but this option does not reflect that.
**Option C:** This option is incorrect because it does not match the base pairing rules. For example, the third nucleotide 'T' in the original sequence should pair with 'A' in the complementary sequence, but this option does not show that.
**Option D:** This option is incorrect because it does not correctly match the base pairing rules. For instance, the fourth nucleotide 'C' in the original sequence should pair with 'G' in the complementary sequence, but this option does not reflect that.
**Clinical Pearl / High-Yield Fact**
Remember to always apply the base pairing rules (A-U, G-C, T-A) when determining complementary RNA sequences. This will help you accurately predict the correct sequence and avoid common mistakes.
**Correct Answer:** A. UACGACTGU