Biochemistry Substitution of which one of the following amino acids in place of alanine would increase the absorbance of proteins at 280 mm: A Leucine B Arginine C Tryptophan D Protein 💡 Explanation Substitution of which one of the following amino acids in place of alanine would increase the absorbance of proteins at 280 mm: ✓ Correct Answer: C. Tryptophan