All of the following statements about hybridoma technology are true, EXCEPT:
Correct Answer: Myeloma cells with mutation salvage pathway grows well in HAT medium.
Description: Hybridomas are cells that have been engineered to produce a specific antibody in huge numbers. To achieve this, qualities, of two types of cells have to be combined together i.e.Cells which can produce large amount of pure antibody, and Cells which have the ability to grow continually. These two types of cells are then fused together to form hybridoma procedure. The pure antibody secreting cells are produced by injecting specific antigen in a mouse and obtaining the antigen specific plasma cells (antibody producing cell) from the mouse's spleen. Cell which can grow indefinitely in culture are myeloma cells. These two cell lines axe fused together. The hybrid cell which is thus produced can be cloned to produce large number of identical daughter clones. These daughter clone cells then produce antibodies. Since these antibodies come from only one type of cell (hybridoma cell) they are called monoclonal antibodies. Ref: Ananthanarayanan & Paniker's textbook of microbiology 7th ed pg 137.
Category:
Microbiology
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