**Core Concept**
Bipolar staining is a technique used to identify bacteria that have a distinctive appearance with two distinct ends, often referred to as "diplococci" or "safety pin" appearance. This staining pattern is typically seen in bacteria that have a thick peptidoglycan cell wall and a small amount of capsular material, such as *Neisseria meningitidis*.

**Why the Correct Answer is Right**
Bipolar staining is particularly characteristic of *Neisseria meningitidis*, *Francisella tularensis*, and *Borrelia burgdorferi*, which all have a distinctive "safety pin" or "diplococci" appearance due to their unique cell wall structure and capsular material. This staining pattern is often used to differentiate these bacteria from other pathogens that may have similar morphologies but lack the characteristic bipolar staining.

**Why Each Wrong Option is Incorrect**
* **Option A:** *Francisella tularensis*, which shows bipolar staining, is a correct example of a bacterium with bipolar staining.
* **Option B:** *Borrelia burgdorferi*, which shows bipolar staining, is a correct example of a bacterium with bipolar staining.
* **Option D:** *Neisseria gonorrhoeae*, which does not typically show bipolar staining, is a correct example of a bacterium that does not show bipolar staining.

**Clinical Pearl / High-Yield Fact**
When interpreting Gram stains or using bipolar staining techniques, it's essential to consider the morphology and staining characteristics of the bacteria to make an accurate diagnosis.

**Correct Answer: D. *Neisseria gonorrhoeae***