**Core Concept**
At physiological pH, amino acids can be separated based on their charge and mobility in an electric field. The isoelectric point (pI) of an amino acid is the pH at which it has no net charge. Amino acids with a pI closer to physiological pH will have a higher charge and migrate faster to the anode.

**Why the Correct Answer is Right**
The amino acid with the highest pI value among the options will be the least charged at physiological pH, resulting in the slowest migration to the anode. This is because the amino acid will be more likely to be protonated and have a positive charge at physiological pH, reducing its mobility.

**Why Each Wrong Option is Incorrect**
**Option A:** This amino acid has a lower pI value, making it more likely to be deprotonated and have a negative charge at physiological pH, resulting in faster migration to the anode.
**Option B:** This amino acid has a similar pI value to the correct answer, but its molecular structure and charge distribution may differ, affecting its migration speed.
**Option C:** This amino acid has a significantly lower pI value, making it highly charged at physiological pH and resulting in rapid migration to the anode.

**Clinical Pearl / High-Yield Fact**
Amino acid separation by charge is a fundamental principle in chromatography and can be applied to various medical and biochemical contexts, such as protein purification and disease diagnosis.

**Correct Answer:** C.