A young male patient presented with urethral discharge. On urine examination pus cells were found but no organisms. Which method would be the best for culture?
Correct Answer: McCoy cell line
Description: Because of absence of organisms in urine examination, the infection is most probably nongonococcal. The most common non gonococcal urethritis is chlamydia urethritis. Chlamydiae grow in cultures of a variety of eukaryotic cells lines. McCoy cells treated with cycloheximide commonly are used to isolate chlamydiae; C pneumoniae grows better in HL or HEp-2 cells. All types of chlamydiae proliferate in embryonated eggs, paicularly in the yolk sac. Ref: Brooks G.F. (2013). Chapter 27. Chlamydia Spp.. In G.F. Brooks (Ed), Jawetz, Melnick, & Adelberg's Medical Microbiology, 26e.
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Microbiology
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