Biochemistry A mutation is MOST likely to alter the three-dimensional conformation of a protein if: A There is a substitution of a hydrophobic amino acid for a hydrophilic amino acid B Valine is substituted for leucine C It changes the amino acid at the amino-terminus D It places proline in the middle of a a-helix 💡 Explanation A mutation is MOST likely to alter the three-dimensional conformation of a protein if: ✓ Correct Answer: D. It places proline in the middle of a a-helix