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529 Pathology

A 45-year-old construction worker suffers a penetrating wound of the left leg, which is cleaned and sutured. Three days later, the patient presents with sudden onset of severe pain at the site of injury. Physical examination shows darkening of the surrounding skin, hemorrhage, and cutaneous necrosis. The wound shows a thick serosanguinous discharge with gas bubbles and a fragrant odor. Which of the following is the most likely etiology of this patient’s wound infection?

AClostridium botulinum
BClostridium perfringens
CStaphylococcus aureus
DStaphylococcus epidermidis
Pathology
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530 Pathology

A pregnant woman with a family history of fragile X syndrome undergoes prenatal testing of her fetus. PCR analysis to amplify the appropriate region of the FMR1 gene is attempted using DNA from amniotic fluid cells, but no amplified products are obtained. Which of the following is the most appropriate next step?

ARoutine karyotyping of the amniotic fluid cells
BRoutine karyotyping of the unaffected father
CSouthern blot analysis of DNA from the amniotic fluid cells
DPCR analysis of the mother's FMR1 gene
Pathology
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