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8175 Microbiology

A 2-year-old child has a fever, stiff neck, and is irritable. Gram stain smear of spinal fluid reveals gram-negative, small pleomorphic coccobacillary organisms. What is the most appropriate procedure to follow in order to reach an etiological diagnosis?

ACulture the spinal fluid in chocolate agar, and identify the organism by growth factors (Correct Answer)
BCulture the spinal fluid in mannitol-salt agar
CPerform a catalase test of the isolated organism
DPerform a coagulase test with the isolate