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6111 Microbiology

23 years old male had unprotected sexual intercourse with a commercial sex worker. Two weeks later, he developed a painless, indurated ulcer on the glans that exuded clear serum on pressure. Inguinal lymph nodes in both groins were enlarged and not tender. The most appropriate diagnostic test is:

AGram's stain of ulcer discharge
BDark field microscopy of ulcer discharge
CGiemsa stain of lymph node aspirate
DELISA for HIV infection
Microbiology
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