For RNA, which blotting technique is used
Correct Answer: Nohern blot
Description: Nohern Blotting for identifying RNA : The Nohern blot is used to demonstrate specific RNA. The total RNA is isolated from the cell, electrophoresed and then blotted on to a membrane. This is then probed with radioactive cDNA .There will be RNA - DNA hybridization. This is used to detect the gene expression in a tissue. Ref :DM.VASUDEVAN.TEXTBOOK ; SEVENTH EDITION ; PAGE NO : 634.
Category:
Biochemistry
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