Which of the following is used in gene therapy?
Correct Answer: Electroporation
Description: Electroporation is used in gene therapy. Gene therapy is a technique that uses genes to treat or prevent disease. Delivery of DNA into patient&;s cells by using recombinant viruses/naked DNA/DNA complexes. Methods used include intranuclear injection, electroporation, gene-gun, sonoporation, and magnetofection Gene therapy was first used for severe combined immunodeficiency disorder (SCID), which is due to adenosine deaminase enzyme deficiency. FDA-approved gene therapy is used for SCID, Leber&;s congenital amaurosis, multiple myeloma and adrenoleukodystrophy Ref: Nelson textbook of pediatrics 21st edition Pgno: 584-586
Category:
Pediatrics
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