Best medium to grow anaerobic bacteria exclusively is: March 2007
Correct Answer: Robeson's cooked meat medium
Description: Ans. B: Robeson's cooked meat medium An anaerobic bacteria culture is a method used to grow anaerobes from a clinical specimen. Robeson's cooked meat medium is probably the most widely used fluid medium for the culture of anaerobes. Obligate anaerobes are bacteria that can live only in the absence of oxygen. Obligate anaerobes are destroyed when exposed to the atmosphere for as briefly as 10 minutes. Facultative anaerobes are those organisms that will grow with or without oxygen. Many anaerobes have characteristic microscopic morphology. For example, Bacteroides spp. are gram-negative rods that are pleomorphic (variable in size and shape) and exhibit irregular bipolar staining. Fusobacterium spp. are often pale gram-negative spindle-shaped rods having pointed ends. Clostridium spp. are large gram-positive rods that form spores. Blood agar is a solid culture medium consisting of agar, peptones and blood. It is an example of an enriched, non selective general purpose medium since it suppos the growth of most aerobic and aerobic bacteria. Sabouraud Dextrose agar is culture medium for the growth of fungi. Thioglycollate medium is a semisolid, nutrient medium that suppos the growth of organisms of all oxygen requirements.
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