Which of the following estimates blood creatinine level most accurately –
Correct Answer: Enzyme assay
Description: Among the given options, the enzyme assay is the most accurate.
Methods to measure serum creatine to assess GFR are:-
i) Gas chromatography-isotope dilution mass spectroscopy (GC-IDMS):- It is the most accurate and method of choice.
ii) High-performance liquid chromatography (HPLC).
(iii) Enzymatic creatinine method (enzyme assay).
(iv)Alkaline picrate methods:- Jaffe method
Accuracy in decreasing order is GC-IDMS > HPLC > Enzyme assay > Alkaline picrate (Jaffe) method.
Category:
Biochemistry
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