The principal site of acidification of urine is
Correct Answer: Collecting duct
Description: The cells of the proximal and distal tubules secrete hydrogen ions. Acidification also occurs in the collecting ducts. The reaction that is primarily responsible for H+ secretion in the proximal tubules is Na+-H+ exchange. Na is absorbed from the lumen of the tubule and H is excreted. Ref: guyton and hall textbook of medical physiology 12 edition page number:504,505,506
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Physiology
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