At Tm or melting temperature, what percentage of dsDNA is denatured?
Correct Answer: 50%
Description: Melting temperature or Tm Temperature when half (50%) of the helical structure is denatured. At lower temperature, the melted strands are re-associated; this is called annealing. When DNA is denatured, unstacking of bases occur resulting in increased absorbance at 260 nm-hyperchromicity
Category:
Biochemistry
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