Investigation of choice for cryptococcosis is –
Correct Answer: India ink
Description: Ans. is 'b' i.e., India ink o C. neoformans is best demonstrated in CSF by direct microscopy. The capsule is seen as a clear halo around the yeast cells in unstained wet preparations of CSF mixed with a drop of India ink or nigrosine. Methenamine silver stain would be the best choice for a tissue sample.CRYPTOCOCCUS NEOFORMANSo The only pathogenic yeasto Four capsular serotypes - A, B, C and Do It has polysaccharide capsuleo Most infections in immunocompromizedpatients are caused by serotype A.o Pigeon droppings commonly contains serotype A and D.o Eucalyptus tree contain serotype B.o It is urease positive.Mode of transmissionBy inhalation of the fungus into the lung (most common)Through skin or mucosa (some times).Laboratory diagnosis# Lumbar puncture is the single most useful diagnositc test.Laboratory diagnosisDirect microscopyCultureSerologyo Unstained wet preperationso Can be easily culturedo The most useful serological testof CSF mixed with drop offrom CSF on sabouroud'sls LPA test (Latex agglutinationIndia ink or nigrosineagartest for the detection of cryptococcaldemonstrate the capsule polysaccharide capsular antigen)as a clear halo o This is highly specific and sensitive; ando Methenamine silver or periodic gives better results than direct microscopyacid-Schiff are used for and cultures.staining a tissue sample.
Category:
Microbiology
Get More
Subject Mock Tests
Practice with over 200,000 questions from various medical subjects and improve your knowledge.
Attempt a mock test nowMock Exam
Take an exam with 100 random questions selected from all subjects to test your knowledge.
Coming SoonGet More
Subject Mock Tests
Try practicing mock tests with over 200,000 questions from various medical subjects.
Attempt a mock test now