Steps of PCR in sequence are?
Correct Answer: Denature DNA, anneal primers, Extends DNA
Description: PCR - method of amplifying a target sequence of DNA* Developed by Karry Mullis in 1984* Essential requirement - 1 targeted DNA 2 Two primers (synthetic oligonucleotides of 17-30 nucleotides length) that are complementary to target DNA.3 Four deoxyribonucleotide4 THERMOSTABLE DNA polymerase * Each cycle has three step (1) Denaturation - The DNA sample is first heat denatured (>90degC) to separate the two strands of the template DNA containing the target sequence(2) Renaturation or annealing -the primers, added in excess, are allowed to anneal to the DNA (typically at 50-75degC) in order to generate the required template-primer complex.(3) Synthesis / extension -he initiation of DNA synthesis occurs at 3'-hydroxyl end of each primer. The primers are extended by joining the bases complementary to DNA strands * Each cycle of PCR takes about 3-5 minutes Three stage of each cycle in relation to temperature and time
Category:
Biochemistry
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