PCR is used to :
Correct Answer: Amplify small amount of DNA
Description: Polymerase chain reaction (PCR) - used to amplify small amount of DNA In 1983, Kary Mullis developed a new technique that made it possible to synthesize large quantities of a DNA fragment without cloning it. Basic Steps involved in PCR are: a) DNA extraction from microorganism b) Amplification of extracted DNA- 3 steps -Denaturation (95degC), Primer annealing (55degC), Extension (72degC) c) Post amplification analysis by different methods like Gel electrophoresis.
Category:
Microbiology
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