Prussian blue reaction is used to demonstrate?
Correct Answer: Hemosiderin
Description: Ans. a (Hemosiderin) (Ref. Harrison medicine 17th ed., Chapter 98 and Figure ell-37)Iron Stores# Prussian blue staining is done for marrow iron stores.- Iron stores can be graded on a scale of 0 to 4+.# A: a marrow with excess iron stores (>4+);# B: normal stores (2-3+);# C: minimal stores (1+); and# D: absent iron stores (0).# Intracellular ferritin is located in both the cytosol and lysosomes, in which partially degraded protein shells of ferritin aggregate into hemosiderin granules.# With a hematoxylin and eosin stain, hemosiderin appears in cells as golden yellow granules.# The iron in hemosiderin is chemically reactive and turns blue-black when exposed to potassium ferrocyanide, which is the basis for the Prussian blue stain.# In iron-overloaded cells, most iron is stored in hemosiderin.Also KnowIRON ABSORPTION# Iron absorption takes place largely in the proximal small intestine.# The function of ferroportin is negatively regulated by hepcidin, the principal iron regulatory hormone.# In the process of release, iron interacts with another ferroxidase, hephaestin, which oxidizes the iron to the ferric form for transferrin binding. Hephaestin is similar to ceruloplasmin, the copper-carrying protein.Sr. Ferritin# Very small amounts of ferritin normally circulate in the plasma.# Its levels correlate well with body iron stores.# During earliest iron deficiency period, iron stores--reflected by the serum ferritin level or the appearance of stainable iron on bone marrow aspirations--decrease.# As long as iron stores are present and can be mobilized, the serum iron, total iron-binding capacity (TIBC), and red cell protoporphyrin levels remain within normal limits. At this stage, red cell morphology and indices are normal.# Gradually, the TIBC increases, as do red cell protoporphyrin levels.# The serum ferritin level is the most convenient laboratory test to estimate iron stores.# By definition, marrow iron stores are absent when the serum ferritin level is <15 pg/L.DIAGNOSIS OF MICROCYTIC ANEMIATestsIron deficiencyInflammationThalassemiaSideroblasticSmearMicro/hypoNormal micro/ hypoMicro/hypo with targetingVariableSI<30<50Normal to highNormal to highTIBC>360<300NormalNormalPercent saturation<1010-2030-8030-80Ferritin (pg/L)<1530-20050-30050-300Hemoglobin patternNormalNormalAbnormalNormalDIAGNOSIS OF HYPOPROLIFERATIVE ANEMIASTestsIron DeficiencyInflammationRenal DiseaseHypometabolicAnemiaMild to severeMildMild to severeMildMCV (ft)60-9080-909090MorphologyNormo-microcyticNormocyticNormocyticNormocyticSI<30<50NormalNormalTIBC>360<300NormalNormalSaturation (%)<1010-20NormalNormalSerum ferritin (mg/L)<1530-200115-150NormalIron stores02-4+1-4+Normal
Category:
Pathology
Get More
Subject Mock Tests
Practice with over 200,000 questions from various medical subjects and improve your knowledge.
Attempt a mock test nowMock Exam
Take an exam with 100 random questions selected from all subjects to test your knowledge.
Coming SoonGet More
Subject Mock Tests
Try practicing mock tests with over 200,000 questions from various medical subjects.
Attempt a mock test now